| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-814 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate |
1989-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1996-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_493656e34f6d9cf82ea32110c32ce32b |
| publicationDate |
1996-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5589386-A |
| titleOfInvention |
Hydrolysis of cholinesterase inhibitors using parathion hydrolase |
| abstract |
A gene is provided encoding a DNA sequence for the expression of parathion hydrolase and a methionine analog thereof. In a preferred embodiment, a parathion hydrolase gene encoding sequence is obtained in which twenty-eight amino acids are deleted from the N-terminal amino acid sequence of parathion hydrolase. Parathion hydrolase or its analog is produced by transforming a host microorganism such as Escherichia, Bacillus or Streptomyces with the gene, culturing the microorganism in a culture medium and purifying parathion hydrolase therefrom. Highly purified soluble parathion hydrolase is produced by a purification method without using detergents such as Triton X-100 and Tween 20. Parathion hydrolase of enhanced activity is produced by adding cobalt or zinc or a mixture thereof to the culture medium in which the host microorganism is cultured. The parathion hydrolase is useful for hydrolyzing organophosphorus compounds such as the cholinesterase inhibitors, diisopropylphosphoro-fluoridate, pinacolylmethylphosphono-fluoridate and isopropylmethylphosphono-fluoridate. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10413769-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8557547-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020064979-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004175407-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010210745-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9796990-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004161818-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3628315-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009238811-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009025107-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011070376-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004109853-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010233146-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8388904-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006286006-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5928927-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8293244-B2 |
| priorityDate |
1988-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |