| abstract |
The present invention provides a method for determining the presence of a class of an antibody in a biological sample. In this method, a first reagent including insoluble particles having an antigen to the antibody immobilized on the surface thereof, and a second reagent including insoluble magnetic particles having immobilized on the surface thereof a substance particularly reactive to a specific immunoglobulin class, is reacted with the sample under conditions to promote agglutination of the first and second reagents with the antibody. The unreacted second reagent and the agglutinate are separated from the unreacted first reagent by application of a magnetic field. Then the amount of unreacted first reagent is determined. |