http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5576433-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7929020d987fe052776931d8b8291e13 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D413-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D413-04 |
| filingDate | 1994-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1996-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b51315f4c78c367cc91e3397fc93afe7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90ab71703edf2f2b9ac54cda3a141fae |
| publicationDate | 1996-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-5576433-A |
| titleOfInvention | Fluorescent calcium indicators targeted to specific intracellular environments |
| abstract | The present invention discloses several new calcium indicator dyes which are retained in the cytosol or which bind to cellular membranes and report calcium levels near the membrane. These indicators are analogs of a previously described tetracarboxylate calcium indicator fura-2 and indo-1 where the 5' methyl of the BAPTA moiety is replaced with a derivative of propionic acid in the form of -CH2CH2CON-(CH2)n-N(X1)X2, where n is 4. The propionic acid derivative provides a flexible means for introducing additional functionality to the calcium indicator without greatly impacting the fluorescence and ion chelating properties of the parental indicator. When X1 is CH2COOH and X2 is part of a ring system like piperazine, a fluorescent calcium indicator, Fura-PE3, is obtained which resists compartmentalization and leakage out of the cytosol. The tertiary amino group survives subsequent acetoxymethyl esterification of the hexacarboxylate form of the indicator without quaternization. In addition, the tertiary amino group can ionize once the indicator is inside the cell giving a zwitterion. All the zwitterionic calcium indicators reported herein resist leakage and compartmentalization. When X1 is a sufficiently hydrophobic alkyl chain, as in C12H25, and X2 is part of a ring system like piperazine, FFP-18, the resulting indicator binds to membranes and reports calcium levels near the membranes. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005233467-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9843082-A1 |
| priorityDate | 1994-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 106.