| abstract |
This invention relates to an improvement of the procedure for amplifying a target nucleotide sequence by using an effective amount of a glycine-based osmolyte in the reaction mixture of an amplification procedure. It has been found that the use of a glycine-based osmolyte reduces the appearance of stutter bands in the amplification product allowing for easier detection of the target nucleotide sequence. For example, detection of the target trinucleotide repeat sequence, indicative of Huntington's Disease, is made clearer with the use of a glycine-based osmolyte. |