| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14effe3850d2129f863b969dac4f1db8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-942
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-395 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
| filingDate |
1993-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1996-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d510981acab80c9414064904aefb1975
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70212f47976c63da984ade0ea5f5adf7 |
| publicationDate |
1996-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5527687-A |
| titleOfInvention |
Enzyme induction screen for ergosterol biosynthesis inhibitors |
| abstract |
A method for screening for sterol biosynthesis inhibitors of potential use as fungicides or antihypercholesterolemic agents identifies agents by the induction of lanosterol 14- alpha -demethylase, an enzyme in the biosynthetic pathway of ergosterol and cholesterol, in cultures containing the agents. In one screening test, test samples are incubated in a culture of a Saccharomyces cerevisiae strain sensitive to ergosterol biosynthesis and containing a gene fusion of a lanosterol 14- alpha -demethylase clone with a gene for bacterial beta -galactosidase. After incubation of the culture, an increase in lancsterol 14- alpha -demethylase activity is determined indirectly by measuring beta -galactosidase activity. The culture media contains a chromogenic substrate of beta -galactosidase such as orthonitrophenyl- beta -D-galactoside or 5-bromo-4-chloro-3-indoyl- beta -D-galactoside, so that active samples are identified by the production of colored product. For comparison purposes, screening tests may employ a lanosterol 14- alpha -demethylase inhibitor such as dinaconazole as a positive control. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005012559-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6630321-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7872115-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011136128-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006088859-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813129-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813129-B |
| priorityDate |
1993-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |