| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03d111d6dcb3d7a07e4e766163463ff9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P13-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-08 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P13-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P43-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
1993-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1996-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61c0661ea6379b54e925b552a343c8eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88c56a3ecbb95fa26a10dd3c6a905437
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b6d67eb934dac2077c5c36e1520f953
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46047ebd7a65e6e4ec1a4a804f1826de
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3dfae5e3c8d30a65b442698c1772d4f |
| publicationDate |
1996-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5512459-A |
| titleOfInvention |
Enzymatic method for modification or recombinant polypeptides |
| abstract |
The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit. The invention utilizes known and newly discovered cleavage recognition sites to effectuate the desired modification products. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005119178-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6849708-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004146985-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7138486-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6037143-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7544512-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6107060-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021198345-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7141659-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004185114-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003207402-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6051399-A |
| priorityDate |
1993-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |