http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5489512-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c5d35cbcd18261ca6afc76a9f64ae2a1 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 1993-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1996-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a822ed23c90287fd7981555d8b0b8f3a |
publicationDate | 1996-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-5489512-A |
titleOfInvention | Method of optimizing a nucleic acid hybridization assay for detection of salmonella in RV growth media |
abstract | A method for enhancing the hybridization signal of a nucleic acid hybridization assay for the DNA or RNA of a Salmonella in a sample by adding the sample to a RV growth medium under conditions sufficient to allow any Salmonella in the sample to propagate, propagating Salmonella, if any, in the medium for a time sufficient to allow the number of Salmonella to reach a predetermined titer, removing trace minerals from the medium, adding a nucleic acid probe to the medium under stringency conditions sufficient to allow the probe to preferentially hybridize with the Salmonella, if any, to form hybridization products that emit an enhanced signal, and assaying the medium to detect the enhanced signal. The method is especially suited for detecting Salmonella in food samples. |
priorityDate | 1991-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.