| abstract |
PCT No. PCT/EP92/01772 Sec. 371 Date Jun. 3, 1994 Sec. 102(e) Date Jun. 3, 1994 PCT Filed Aug. 5, 1992 PCT Pub. No. WO93/11174 PCT Pub. Date Jun. 10, 1993.Matrices for electrokinetic and chromatographic separations, based on a unique class of mono- and disubstituted (on the amido nitrogen) acrylamides (e.g., acrylamido-N-ethoxy ethanol, acrylamido-N,N-diethoxy ethanol) off erthe following advantages: a) strong resistance to the alkaline hydrolysis (most zone separations occurring at basic pH values); b) a high hydrophilicity; and c) greater porosity, due to the larger Mr value of the monomers. Such matrices can be prepared as 'chemically crosslinked' gels or as 'physical' gels, the latter consisting of strings of monofunctional monomer in the absence of crosslinker, at concentrations above the entanglement threshold. It is moreover possible to further increment the gel porosity by polymerizing it in the presence of laterally aggregating agents (e.g., polyethylene glycol of Mr 6-20,000 Da), thus obtaining matrices which allow better resolution of large Mr proteins and of nucleic acid fragments; by polymerizing the matrix in the presence of a gradient of the laterally aggregating agent, it is possible to obtain a porosity gradient even at a constant percent of monomers in the gel. Finally, photopolymerization (e.g., with methylene blue and a redox couple, sodium toluene sulfinate and diphenyliodonium chloride and light), instead with the standard red/ox catalysts, prevents formation of N-oxides of amines present in the polymerizing solution. |