| abstract |
A method for determining if tissue is malignant as opposed to non-malignant (i.e., benign tumor tissue, benign tissue, or normal tissue). In one embodiment, the method comprises irradiating a human breast tissue sample with light at a wavelength of about 310 nm and measuring the time-resolved fluorescence emitted therefrom at about 340 nm. The time-resolved fluorescence profile is then compared to similar profiles obtained from known malignant and non-malignant human breast tissues. By fitting the profiles to the formula I(t)=A1e(-t/ tau 1)+A2e(-t/ tau 2), one can quantify the differences between tissues of various conditions. For example, non-malignant human breast tissues exhibit a slow component ( tau 2) which is less than 1.6 ns whereas malignant human breast tissues exhibit a slow component ( tau 2) which is greater than 1.6 ns. In addition, non-malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A1/A2) which is greater than 0.85 whereas malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A1/A2) which is less than 0.6. This technique can be used with different excitation and/or emission wavelengths, and can be applied to the detection of malignancies (or other abnormal states) in tissues other than human breast tissue. |