| abstract |
A dry multilayer analytical element for assaying transaminases wherein the analytical element comprises: a) a transaminase substrate; b) reagents that generate pyruvate in the presence of a transaminase and the transaminase substrate; c) pyruvate oxidase and cofactors for pyruvate oxidase; and d) reagents that generate a color in proportion to a concentration of hydrogen peroxide; and wherein the analytical element has, in the following order, a spreading layer, a reagent layer, and a support wherein i) the spreading layer contains the transaminase substrate and a material selected from the group consisting of polymer beads, barium sulfate, titanium oxide, and mixtures thereof; ii) the reagent layer contains the pyruvate oxidase; and iii) both a hardened gelatin layer and a subbing layer separate the reagent layer from the spreading layer. |