| abstract |
PCT No. PCT/FR92/00832 Sec. 371 Date Feb. 25, 1994 Sec. 102(e) Date Feb. 25, 1994 PCT Filed Aug. 28, 1992 PCT Pub. No. WO93/05049 PCT Pub. Date Mar. 18, 1993.The invention relates to macrocyclic rare earth complexes which consist of at least one rare earth salt complexed by a macrocyclic compound of formula (I): <IMAGE> (I) in which: the bivalent radicals +E,crc/A/ , +E,crc/B/ , +E,crc/C/ and +E,crc/D/ , which are identical or different, are hydrocarbon chains optionally containing one or more heteroatoms, at least one of said radicals containing at least one molecular unit or essentially consisting of a molecular unit possessing a triplet energy greater than the energy of the emission level of the complexed rare earth ion, at least one of said radicals consisting of a substituted or unsubstituted nitrogen-containing heterocyclic system in which at least one of the nitrogen atoms carries an oxy group, and it being possible for one of the radicals +E,crc/C/ or +E,crc/D/ not to exist; and X1 and X2, which are identical or different, are hydrogen or a hydrocarbon chain (CH2)n optionally interrupted by 1 or more heteroatoms, n being an integer from 1 to 10, with the proviso that if the radicals +E,crc/A/ and/or +E,crc/B/ are a nitrogen-containing heterocyclic system in which at least one of the nitrogen atoms carries an oxy group, the radicals +E,crc/C/ and/or +E,crc/D/ are selected from biquinolines, biisoquinolines, bipyridines, terpyridines, coumarins, bipyrazines, bipyrimidines and pyridines, and to their use for reducing the perturbations in a fluorescent assay. |