http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5449615-A
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01129 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01018 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
filingDate | 1994-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1995-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1aaa661cb34def812ecd3c23d1834008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a8d9108df1c02207f979acdff68659d |
publicationDate | 1995-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-5449615-A |
titleOfInvention | KDN-cleaving sialidase isolated from hepatopancreas of mollusks |
abstract | A sialidase capable of cleaving a glycoconjugate to yield 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) is isolated from hepatopancreas of mollusks. Preferably, the sialidase is isolated by processing crude oyster hepatopancreas to form a crude extract of the sialidase enzyme. The crude enzyme is further concentrated and refined utilizing sequential column chromatography fractogel EMD DEAE-650 (M), sephacryl S-200, and fractogel EMD SP 650 (M) chromatography, respectively. Using this procedure, the sialidase is purified over 155 fold with a 17% recovery. Ferric ion exerts a three fold stimulation in the activity of the sialidase, at a concentration of 3 mM. The sialidase cleaves 4-methylumbelliferyl-KDN, KDNα2→3Galβ1→4GlcCer, KDNα2→6Galβ1→4GlcCer, or KDNα2→6GalNAc-o1 to yield KDN. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6001635-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6136552-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5834288-A |
priorityDate | 1994-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 213.