| abstract |
PCT No. PCT/JP93/00586 Sec. 371 Date Jan. 3, 1994 Sec. 102(e) Date Jan. 3, 1994 PCT Filed Apr. 30, 1993 PCT Pub. No. WO93/22448 PCT Pub. Date Nov. 11, 1993.In a process of production of a useful protein by culturing a human embryonal kidney cell-derived 293 strain which is introduced with a useful protein-expressing gene, it becomes possible to obtain a culture fluid containing the useful protein in a high concentration using an extremely small amount of the medium, by carrying out a fed-batch culture and adding a sugar or a sugar and a calcium in a specified stage of the culture. |