patent/US-5436150-A

http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5436150-A

Outgoing Links

Predicate	Object
assignee	http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb
classificationCPCInventive	http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
classificationIPCAdditional	http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01
classificationIPCInventive	http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate	1993-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate	1995-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor	http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_094242f31207bc3db82d44a756d12d12
publicationDate	1995-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber	US-5436150-A
titleOfInvention	Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
abstract	The present inventors have identified the recognition and cleavage domains of the FokI restriction endonuclease. Accordingly, the present invention relates to DNA segments encoding the recognition and cleavage domains of the FokI restriction endonuclease, respectively. The 41 kDa N-terminal fragment constitutes the FokI recognition domain while the 25 kDa C-terminal fragment constitutes the FokI cleavage nuclease domain. The present invention also relates to hybrid restriction enzymes comprising the nuclease domain of the FokI restriction endonuclease linked to a recognition domain of another enzyme. One such hybrid restriction enzyme is Ubx-FN. This enzyme contains the homeo domain of Ubx linked to the cleavage or nuclease domain of FokI. Additionally, the present invention relates to the construction of two insertion mutants of FokI endonuclease.
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