| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0085
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-99009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate |
1992-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1995-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75d54a87c14a39b301ccbeda1761295e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_601ed7893bf698c5cc55d3ecd466e9f2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a862fc75cfbd10d67914d32d0c26b50 |
| publicationDate |
1995-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5420027-A |
| titleOfInvention |
Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria |
| abstract |
Disclosed are methods and compositions for effecting bacterial expression of eukaryotic cytochrome P450 enzymes and fusion proteins comprising a eukaryotic P450 domain fused to a reductase enzyme domain in a biologically active form. Certain embodiments involve the expression of eukaryotic cytochrome P45017 alpha -hydroxylase which is expressed in large amounts in an E. coli host in a biologically active form without the need for coexpression or admixture of a cytochrome P450 reductase. Methods and compositions are also disclosed for the construction of cytochrome P450 enzyme hybrids or fusion proteins, incorporating the N-terminal 9 amino acids from bovine 17 alpha -hydroxylase, which will enable many eukaryotic cytochrome P450 enzymes or fusion proteins to be expressed in bacteria. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2279754-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2279755-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2279753-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007122865-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6492316-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9913106-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6929912-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003215915-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9255256-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6566108-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006183209-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5952211-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112725296-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2338333-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2305314-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2055189-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2042196-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100665316-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2080525-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003096251-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6432639-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-I407264-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2322229-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003059800-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2305311-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2305312-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2305313-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2298354-A2 |
| priorityDate |
1991-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |