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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f83fb720d5620a58eded1697b0c7f37a
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publicationDate 1995-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5411886-A
titleOfInvention Xylose isomerase gene of Thermus aquaticus
abstract A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus characterized in that the xylose isomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95 DEG C. and the molecular weight of about 44,000, and is stabilized with manganese or magnesium. A process for preparing a xylose isomerase comprising transforming a microorganism with a plasmid containing the above gene and a promoter, culturing the transformed microorganism and harvesting the produced xylose isomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xylose isomerase.
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