| abstract |
Two types of structural analogues of D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P 4 ] are prepared by a chemo-enzymatic route. These 6-O-substituted analogues retain the biological activity of Ins(1,4,5)P 3 and are able to elicit Ca 2+ release from porcine brain microsomes. Moreover, these analogues allow the preparation of Ins(1,4,5)P 3 -based immunogens and affinity matrix which are successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P 3 . These antibodies display discriminative affinity toward Ins(1,4,5)P 3 , and provide a useful tool to study intracellular Ca 2+ mobilization. |