| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4bef96a52ca9eaf6230a857ba74af9a1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-825 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 |
| filingDate |
1992-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1995-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e37fdd303da393d259019e969e4f1a4e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09f7ad52b342947690998f5746985123 |
| publicationDate |
1995-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5391715-A |
| titleOfInvention |
Method for isolating and purifying bordetella pertussis antigenic factors |
| abstract |
A process is provided for the purification of pertussis toxin (PT) and/or filamentous hemagglutinin antigen (FHA) from a B. pertussis fermentation broth or cell free culture supernatant containing at least one antigen, which comprises contacting the fermenation broth or cell free culture supernatant with a hydroxyapatite adsorbent for a sufficient time to adsorb the antigen(s) at a pH which both PT and FHA are adsorbed and eluting a mixture containing the adsorbed antigen(s) from the adsorbent with eluant at a pH which both PT and FHA are eluted. The PT and FHA may be further purified by two sequential chromatographic columns, one of which involves apolar-ligand chromatography. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104906569-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010330124-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8647640-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007020292-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8137935-B2 |
| priorityDate |
1989-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |