Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07c9542186133097bd6bbb7d92393090 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-814 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-804 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78 |
filingDate |
1991-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1994-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_955769b4d23bf68cfce475da17a1daae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_616eb3536037856acffe147a785b1325 |
publicationDate |
1994-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-5342762-A |
titleOfInvention |
Fibronectin purification vector |
abstract |
Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin containing affinity column and digested with a protease such as trypsin to cleave the desired protein from the gelatin binding region. The vectors can also be designed to code for factor XIIIa cross liking sites and to have a chemically reactive cysteine residue. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003228284-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1173590-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003050470-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0049159-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004053368-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5460955-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9616179-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7071172-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0116289-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9964631-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03093295-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03093295-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6218529-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0116289-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005014242-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1173590-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6420157-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002102709-A1 |
priorityDate |
1991-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |