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filingDate 1991-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1994-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1994-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5342762-A
titleOfInvention Fibronectin purification vector
abstract Recombinant protein purification vectors and methods for their use are disclosed. The vectors contain a DNA sequence coding for a gelatin binding region of fibronectin. The vectors express a foreign DNA sequence of interest fused to the fibronectin portion. Secretion signals on the fused product assist the product in being secreted from a production cell. The product can then be purified on a gelatin containing affinity column and digested with a protease such as trypsin to cleave the desired protein from the gelatin binding region. The vectors can also be designed to code for factor XIIIa cross liking sites and to have a chemically reactive cysteine residue.
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6420157-B1
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