| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f8565c8b0315a5c1678dca65ddfbc47a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M1-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-0613
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M21-18 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
| filingDate |
1991-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1994-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f26d5192cc7327bbbcff8b6e790bfd8 |
| publicationDate |
1994-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5336601-A |
| titleOfInvention |
Enzymatic membrane method for the snythesis and separation of peptides |
| abstract |
The present invention discloses a method for the enzymatic synthesis of a peptide. A protected peptide having a C-terminal carboxylate group or a protected, N-acyl amino acid having an alpha carboxylate group is reacted with a protected peptide having an N-terminal ammonium group or a protected amino acid having an alpha ammonium group in the presence of a condensation enzyme under conditions in which the carboxylate group and the ammonium group condense to form a protected, uncharged, peptide product. This peptide product is transported across a water-immiscible hydrophobic phase into an aqueous product phase. This peptide product is removed from the aqueous product phase to prevent back diffusion across the water-immiscible hydrophobic phase. Reverse osmosis and other separation techniques may be utilized to remove the peptide product. The water-immiscible hydrophobic phase is an ion rejection membrane separating the aqueous reaction phase from the product phase creating oil/water interfaces with each of the aqueous phases. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003039868-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006219634-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004110602-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5795750-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03045981-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7316728-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03045981-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6500571-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005010056-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004234944-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100431674-C |
| priorityDate |
1986-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |