http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5330904-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-948 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
| filingDate | 1992-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1994-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6df92260a4fe201f89e0988d7e5b66a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19361ed42bd447d714c1f20725c07049 |
| publicationDate | 1994-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-5330904-A |
| titleOfInvention | Method for mammalian cell transfection using 25-hydroxycholesterol |
| abstract | The present invention relates to a process, a cell culture medium, and a kit for the rapid, high-efficiency transfection of mammalian cells with exogenous DNA. The process comprises incubating a cell culture in the presence of a transfection medium comprising a serum that is different from the serum in the normal growth medium used to grow the cells, and DNA to produce transfected cells. In a preferred embodiment the normal growth medium comprises fetal bovine serum and the transfection medium comprises a serum such as human, calf, horse, lamb, or pig serum. The transfection medium may further comprise an hydroxylated sterol such as 25-hydroxycholesterol. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5589368-A |
| priorityDate | 1992-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.