| abstract |
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, serum-free supplements for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin fraction mitogen, epidermal growth factor and insulin are described. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC, which can be transplanted to muscles of patients afflicted with muscle degenerative diseases such as muscular dystrophy. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC. |