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filingDate 1991-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1994-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0851177ba48525491b3247c254364e9
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publicationDate 1994-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5304484-A
titleOfInvention Non-hemolytic mosquitocidal microorganisms
abstract The cyta gene encoding the 28 kDa polypeptide of Bacillus thuringiensis israelensis crystals was disrupted in the 72 MDa resident plasmid by in vivo recombination. The absence of the 28 kDa protein in B. thuringiensis israelensis did not affect the crystallization of the other toxic components of the parasporal body. However, the absence of the 28 kDa protein did abolish the hemolytic activity of B. thuringiensis serovar israelensis crystals. The mosquitocidal activity of the 28 kDa protein-free crystals did not differ significantly from the wild-type crystals.
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