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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
filingDate 1992-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1994-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e66d2d6d63e0a47065c1fa4a86facbe8
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publicationDate 1994-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5286636-A
titleOfInvention DNA cloning vectors with in vivo excisable plasmids
abstract Vectors are described that circumvent traditional DNA cloning and subcloning procedures, and that contain a unique DNA cartridge that permits both cloning of DNA directly into DNA sequences present within the cartridge, and in vivo removal and circularization of the cartridge thereby yielding an autonomously replicating structure. Because the DNA cartridge can include a wide variety of functional DNA sequences, the cloned DNA can be subjected to a plethora of molecular biological procedures without having to remove the cloned DNA from the cartridge thereby obviating the need to perform additional subcloning techniques. A particularly useful example of this type of vector is bacteriophage lambda containing the DNA cartridge.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2332975-A1
priorityDate 1987-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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