| abstract |
PCT No. PCT/HU89/00021 Sec. 371 Date Jun. 3, 1991 Sec. 102(e) Date Jun. 3, 1991 PCT Filed May 12, 1989 PCT Pub. No. WO90/08317 PCT Pub. Date Jul. 26, 1990.A method for the selective determination of sialic acid-carrying glycolipid complexes in blood, in which the serum is diluted with water, then extracted with a water-immiscible organic solvent or solvent mixture to remove neutral lipids, a lipoprotein fraction containing sialic acid-carrying glycolipids is precipitated from the aqueous phase, the precipitated fraction is redissolved, and the sialic acid content of the solution or the amount and quality of the sialic acid-carrying glycolipid are determined by a method known per se. A water-soluble salt of a monovalent, bivalent or trivalent metal other than alkali metal is used as a precipitating agent in an amount corresponding to a final metal ion concentration of 0.005-0.1 mole/l in the mixture, and precipitation is performed at a pH of 1.5 to 8.0. |