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filingDate 1991-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1993-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e5cdd892ab33f233e579d11fb89dbc1
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publicationDate 1993-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5270788-A
titleOfInvention Apparatus for measuring polarization of bathochromically shifted fluorescence
abstract An improved method and apparatus for measuring polarized fluorescence emissions compensates for background emissions without separating a fluorescing material from background material. The method involves measuring the horizontally and vertically polarized components of the fluorescence emission at a primary wavelength and at least one secondary wavelength selected on the basis of the bathochromic shift of the spectrum of the fluorescence emissions from the fluorescing material as compared to the fluorescence emissions from the background. From these measurements, a factor representing the fraction of the total intensity of fluorescence emissions due to background fluorescence is determined. From this factor, the intensities of the vertically and horizontally polarized fluorescence emissions due to background fluorescence are derived and subtracted from the measurements at the primary wavelength to obtain intensities due solely to the material being analyzed.
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priorityDate 1986-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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