| abstract |
Asymmetrically tailed plasmid primers are provided, each of which comprises a cut, double-stranded DNA plasmid containing a functional origin of replication, at least one functional selection marker gene, a 3' oligo (dT) tail and a 3' oligo (dC) or oligo (dG) tail which is terminated by a phosphate group. Methods for making and using the primers for the highly efficient production of complex cloning libraries and a kit for carrying out the cloning method of the invention are also provided. |