| abstract |
The present invention discloses a recombinant DNA molecule having a full length or a truncated T5 DNA polymerase structural gene, each encoding a processive, thioredoxin-independent DNA polymerase. The DNA polymerase of the invention may have 3'-to-5'exonuclease activity or may be substantially reduced in processive 3'-to-5'DNA exonuclease activity. A method for producing these enzymes is also disclosed, as is the proteins produced by this process. The present invention is also directed to a leader sequence important for expression of soluble T5 DNA polymerase protein. |