| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1721d75f5bf603447ec5a26b5c1c0be0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10122 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
1990-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1993-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_784a1b20dcea8b31705239bf369e8f26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_886c97c101f89820aae96bb9e008d2ff |
| publicationDate |
1993-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5229273-A |
| titleOfInvention |
Purified bacterial protein which stimulates ligation of nucleic acid molecules |
| abstract |
The subject invention provides a purified protein having a molecular weight of about 22.8 kDa and the amino acid sequence shown in FIG. 2. The invention further provides a method of producing the 22.8 kDa protein which comprises culturing the host cell comprising an expression vector under conditions such that the expression vector contained therein expresses the protein and recovering from the host cell the protein so expressed. Also provided is a method of ligating separate nucleic acid molecules which comprises contacting the molecules with the 22.8 kDa protein under conditions such that the molecules are ligated together. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8785157-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6551618-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450608-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10190164-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007231823-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9657291-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9611470-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012021803-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9752133-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9309560-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7993842-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9540637-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9822395-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006024681-A1 |
| priorityDate |
1990-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |