| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2de1389433aab193063aa0a2c8c693d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6481 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate |
1992-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1993-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56c49daa9181f03172364c12297e09c1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e80fca1bdb2aefbf6e6d0e488b3c8d9 |
| publicationDate |
1993-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5215908-A |
| titleOfInvention |
Process for recovery and purification of chymosin |
| abstract |
This invention provides a method of recovering and purifying chymosin from fermentation used for microbial production of chymosin. The fermentation mixture is filtered to provide a filtrate containing the chymosin. The filtrate is first prepared by either adjusting it to a low pH, such as about 2 and/or adjusting the salt concentration of the filtrate to at least about 2M. The aqueous filtrate mixture is contacted with a phenyl-sepharose resin, which selectively binds the chymosin from the filtrate. The filtrate remaining after the contact with the phenyl-sepharose resin is discarded, and the phenyl-sepharose resin column is eluted with water or dilute salt solution to obtain substantially pure chymosin in a single elution step. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008131487-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004219613-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002160445-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7390936-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008184394-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7682793-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5801034-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1362099-B2 |
| priorityDate |
1989-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |