| abstract |
The process for the preparation of the biologically active subunit ( beta subunit) of human nerve growth factor (NGF) from human placenta is characterized by the steps of: a) homogenization of the tissue of human placenta, b) dialysis at pH 4.0 in order to dissociate the 7S NGF form, and c) chromatographic fractionation of the resultant solution on a three step cation exchange chromatography column, d) purification of the active fraction obtained by cation exchange chromatography with a gradient of sodium chloride and pH. e) purification of the active fraction thus obtained by chromatography on polyclonal antibodies, raised against mouse NGF. t |