| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_782b276beee7c34efe50183392517dfb |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-60 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-60 |
| filingDate |
1991-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1993-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e21904c19ede28a7d75bbc0f1147369e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9b9161da86d059726100bfaac8cc029
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5cc90508ff36a072d7d62d53dc91c49
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82c55a11706aae78bba5b5b6c89e32ce |
| publicationDate |
1993-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5208332-A |
| titleOfInvention |
Optical probe for the cytochrome P-450 cholesterol side chain cleavage enzyme |
| abstract |
An optical probe enables the study of enzyme activity by absorbance spectroscopy or by sensitive fluorescence methods. In particular, the probe provides the ability to monitor the activity of cytochrome P-450 scc enzyme, the rate limiting enzyme for steroid biosynthesis. Located on the inner mitochondrial membrane, P-450 scc catalyzes the conversion of cholesterol to pregnenolone and isocapraldehyde by sequential oxidations of the cholesterol side chain. The fluorogenic probe includes a cholesterol-like steroid linked to a chromophore through a linking group. The chromophore is selected to have little optical response when linked to the steroid substrate and an enhanced optical response when cleaved from the substrate and linking group. Thus, a fluorescent anion that can be optically detected is generated by the side-chain cleavage reaction during steroidogenesis. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007072256-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7132252-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007110672-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005065241-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6514687-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003186349-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6420130-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009247984-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6414152-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8029765-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010105095-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009004108-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6638713-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8008088-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8153828-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005065241-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8466286-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6143492-A |
| priorityDate |
1991-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |