| abstract |
A method for creating a propagatable, new combinant cell with differentiated function to replenish dwindling number of similar cells in diseased subjects. The new combinant cell is obtained by 1) removing a nucleated cell, which is of the same differentiative phenotype as the diseased cells, from a subject and isolating therefrom a karyoplast, 2) isolating from a source other than said subject a proliferation-prone cell which is of the same differentiative phenotype or which is predestined to become the same phenotype as the diseased cells and isolating therefrom a cytoplast, 3) fusing the cytoplast and karyoplast to form a propagatable, new combinant cell, 4) propagating the combinant cell in culture, and 5) transplanting the combinant cells into said subject. In another embodiment the karyoplast donor is histocompatible to said subject. In yet another embodiment a whole cell is used in lieu of a karyoplast for fusion with the cytoplast. |