| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2478cfab712ee95c8080245b72ecd143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98a74c7179f7e6b5314930688849d7bd |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6421
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6445 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate |
1988-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1992-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fca4d88752037a1cf609f7bdf3155237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e5cdd892ab33f233e579d11fb89dbc1 |
| publicationDate |
1992-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-5166052-A |
| titleOfInvention |
Method for measuring polarization of bathochromically shifted fluorescence |
| abstract |
An improved method for measuring polarized fluorescence emissions compensates for background emissions without separating a fluorescing material from background material contributing background fluorescence. The method is particularly useful in measuring fluorescence from a suspension of cells such as stimulated lymphocytes in the SCM assay, where the intracellular fluorescence is due to the penetration of the cells by a fluorogenic agent precursor and its subsequent hydrolysis. The method involves measuring the horizontally and vertically polarized components of the fluorescence emission at a primary wavelength and at least one secondary wavelength. The secondary wavelength is selected based on the bathochromic shift of the spectrum of the fluorescence emissions from the fluorescing material as compared to the fluorescence emissions from the background. From these measurements a factor representing the fraction of the total intensity of fluorescence emission due to background fluorescence at the primary wavelength is determined, from which the intensities of the vertically and horizontally polarized fluorescence emissions due to background fluorescence are derived. These derived background intensities are then subtracted from the vertically and horizontally polarized fluorescence emission intensities measured at the primary wavelength to obtain intensities due solely to the material being analyzed. More than one secondary wavelength can be used to increase the accuracy of the method. Apparatus suitable for practicing this method is described. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011206291-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1151280-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2019079063-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8649008-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6674527-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5387527-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6379884-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11002655-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1151280-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11747257-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002159056-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108780027-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11199486-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0969279-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8581213-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11187638-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002127591-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8760645-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11435275-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6448018-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0969279-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10908139-B2 |
| priorityDate |
1986-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |