Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69c91bbf0395db010b4806f50d808c4e |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
1988-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1992-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12a9a2f5b89b36ebd51769e8e5a3698c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d421f9b5668cd46487fdeccff798be4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83bae0fb684bc1c7c28cd98e6ee99972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59ca771ded6ad8c165637771051056d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d49b48634c2cd1160600ad0799eb787 |
publicationDate |
1992-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-5160489-A |
titleOfInvention |
Expression vectors and method for their construction |
abstract |
PCT No. PCT/HU88/00061 Sec. 371 Date Mar. 16, 1990 Sec. 102(e) Date Mar. 16, 1990 PCT Filed Sep. 14, 1988 PCT Pub. No. WO89/02466 PCT Pub. Date Mar. 23, 1989.The invention relates to new expression vectors. More particularly, the invention relates to expression vectors carrying a new type of promoters for the expression of the foreign gene, which was constructed from promoter P2 of E. coli ribosomal RNA operon and some regulatory sequences of E. coli las operon. The invention further relates to a method for the construction of the above vectors. The method is characterized by deleting most part of the structurgenes of E. coli ribosomal RNA operon (rrn operon) built in a suitable vector, joining together the part of operon rrn comprising promoter P2 with the beginning part of the lac operon outside the regions -35 and -10, downstream of them so as to create a new NsiI cleavage site at the joining point of the sequences originating from rrn and lac operons, deleting the inner TGCA sequence-element of the newly created NsiI cleavage site and building the structurgene to be expressed between these newly created regulatory sequences. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005100586-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005227326-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7390636-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5763270-A |
priorityDate |
1987-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |