http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5087559-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6115b7f4561db088f26a575e9339fed0 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1988-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1992-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e08d82899ae0806ec5e8d18c56d7cb24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4f0829cb740cb104e5dc7cf48a5d1e7 |
| publicationDate | 1992-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-5087559-A |
| titleOfInvention | Method of detecting single base substitutions in nucleic acids |
| abstract | A method for detecting point mutations or base substitutions in a nucleic acid polymer is especially useful for detecting such mutations in the highest melting domain (HMD) of a double-stranded nucleic acid polymer and is particularly suited for use with RNA. The method involves the steps of: (a) preparing a solution containing a double-stranded nucleic acid polymer comprising a duplex of a single-stranded nucleic acid polymer to be analyzed and a complementary portion of a corresponding wild-type nucleic acid polymer; (b) placing an aliquot of the solution of the double-stranded nucleic acid polymer into each of a series of containers having a range of denaturing conditions, said series including at least one container in which the denaturing conditions are such that a mismatched nucleic acid duplex having one or more base-pair mismatches within its HMD would be denatured to single-stranded nucleic acid polymers, but in which a perfectly matched nucleic acid duplex having no base-pair mismatches within its HMD would not be so denatured; and (c) comparing the occurrence of single stranded nucleic acids in each container after incubation with a standard. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9521268-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5633129-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0168919-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9525815-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0168919-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5856192-A |
| priorityDate | 1988-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.