abstract |
A feedstock containing a biomass such as lignocellulosic materials, e.g. forest biomass; agricultural residues; or manures, is pretreated and thereafter is fractionated into cellulose, lignin and hemicelluloses. New mutants are disclosed which include Chaetomium cellulolyticum IAF-101 (NRRL 18756), Aspergillus sp. IAF-201 (NRRL 18758), Penicillum sp. IAF-603 (NRRL 18759), and Trichoderma reesei QMY-1. With these new mutants and also known fungi including Pleurotus sajor-caju and other Pleurotus spp. unfractionated predetermined biomass is converted into feed. The same treatment can also be applied to hemicelluloses, and cellullose. Cellulose can also be hydrolyzed by means of a cellulase-system prepared from cellulose and Tricoderma reesei to prepare glucose which can be converted to alcohol with Saccharomyces cerevisiae, Kluyveromyces spp. and Zymomonas mobilis. The residual microbial biomass of these microorganisms from alcohol fermentation broth is also used as feed. The process is economical and non polluting. |