Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56ee5285e8e8edccb061046ff20da7a6 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-823 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-525 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 |
filingDate |
1987-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
1991-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9693333ab46e2a4a73a12c9df43c59a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99ccfd622d1469b51f8c0e021d0e72e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc182fa7b2d752f8ffe69f3f57858f7a |
publicationDate |
1991-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-5037736-A |
titleOfInvention |
Process and test carrier for the determination of an analyte |
abstract |
The present invention provides a process for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed. The process contains a step in which the binding components are incubated with one another so that binding reaction takes place. The amount of enzyme-labelled binding component not bound to the carrier-fixed binding component is a measure of the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal. During the specific binding reaction, incubation is carried out simultaneously with a non-fixed substrate of the labelling enzyume which does not produce a detection signal and with a carrier-fixed substrate of the labelling enzyme which produces a detection signal, the substrate not producing a detection signal being so chosen with regard to the amount used and affinity to the labelling enzyme in relation to the amount of the substrate producing a detection signal and its affinity to the labelling enzyme and in relation to the total activity of the labelling enzyme that the enzyme-catalyzed reaction of the substrate producing a detection signal is delayed until the specific binding reaction between the binding components has substantially taken place. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003032196-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1824991-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7605004-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8343726-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9215863-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008096189-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1417491-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1824991-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1417491-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5591645-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006058286-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5271895-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009253119-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9903864-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8003407-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004157281-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5290678-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE38430-E http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8541242-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5232663-A |
priorityDate |
1986-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |