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filingDate 1988-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1991-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1991-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5013527-A
titleOfInvention Integral multilayer analytical element for analysis of albumin
abstract In an integral multilayer analytical element for the analysis of albumin where at least one hydrophilic polymer binder layer and a porous spreading layer containing an indicator capable of binding to albumin to produce a color change are integrally superposed on a light-transmissive water-impermeable support in this order, the improvement comprising said indicator being dispersed in a hydrophilic polymer or a mixture of a hydrophilic polymer and a hydrophobic polymer and a pH buffer being incorporated into the spreading layer. n In the analytical element of the invention, the error caused by the competitive inhibition by globulin can be removed by making to produce the color due to the bonding between albumin and indicator relatively much in the support side (lower side) portion of the porous spreading layer and by making to produce the color due to the bonding between globulin or other proteins and indicator relatively much in the outer surface side (opposite side to the support, upper side) portion of the porous spreading layer.
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