| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c163c101399a77181408b93dc02fe00d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-935 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0028
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13 |
| filingDate |
1987-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1990-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f39ea0fbc104109f5aa75a5f6b61b83b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_704d1bd1abccfd815e056daad690f1c7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ea7d386cba36358174648cda3bf1548
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b159feeda9e8c5cc01713b7484cd3ce3 |
| publicationDate |
1990-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-4959327-A |
| titleOfInvention |
Vectors and method of penicillium chrysogenum transformation |
| abstract |
Disclosed is a method for transforming Penicillium chrysogenum. More particularly, the method includes obtaining an auxotrophic mutant of P. chrysogenum and employing an exogeneous segment of P. chrysogenum DNA capable of complementing said auxotorph so as to restore prototrophy. The exogeneous DNA segment thus comprises a phenotypic marker indicating successful transformation of the mutant. The exogeneous complementary DNA segment may further be prepared in a recombinant plasmid vector. These plasmid vectors include, by way of example, the pPctrpCL and pPctrpC6 plasmids developed by Applicants. These transforming plasmid vectors and those having identifying characteristics thereof are suitable for use in the subject transformation process. The exogeneous complementary DNA segment comprises a gene encoding a selected biosynthetic enzyme. By way of example, these genes include trpC, pyr4, argB and NO-reductase, as well as other genes which encode metabolically required enzymes. The most preferred of these is trpC. |
| priorityDate |
1986-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |