| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-829
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-814
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-962 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-80 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate |
1987-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1990-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_469b5cdc596b377b13721d0b5d044849 |
| publicationDate |
1990-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-4959308-A |
| titleOfInvention |
Immunoassay for antibodies binding platelets |
| abstract |
The present invention involves a method for producing a substrate useful in a system for the detection of antibodies directed against platelet antigens. This method comprises several steps. A platelet sample of interest is initially treated with an aqueous solution comprising a dialyzable nonionic detergent. This initial treatment is under conditions to solubilize platelet components and produce a platelet lysate. Such conditions may involve treatment of a platelet sample with an aqueous solution comprising nonionic detergent at a concentration between about 0.2% and about 0.5%. Platelet antigens are most preferably solubilized for about 30 min and at about 0 DEG C. in an aqueous solution comprising about 1 mg dialyzable nonionic detergent per mg platelet protein. The partially purified platelet antigens resulting from these manipulations are then preferably affixed to a solid matrix. The solid matrix preferably comprises nitrocellulose paper, polystyrene or latex but may be any solid matrix suitable for the abstraction from a biological sample and/or assay of antibodies binding to the affixed purified platelet antigens. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5198357-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9532426-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5223397-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5288610-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9313784-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0184150-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9221978-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5759774-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5427939-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5798216-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8592224-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113721033-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008138823-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003124583-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5482841-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112710846-A |
| priorityDate |
1987-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |