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publicationDate 1990-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4933286-A
titleOfInvention Purification of apase-11 and retrieval of the nematode resistance gene
abstract An isoenzyme from tomato, acid phosphatase-1 isoenzyme (Apase-1 1 ), has ben purified to homogeneity and is subjected to amino acid sequencing and used to prepare anti-Apase-1 antibodies. The amino acid sequence permits design of probes to recover Apase-1 1 -encoding cDNA; the antibodies are also useful for this purpose. The cDNA is useful to recover the genomic DNA encoding Apase-1 1 , which can then be used in walking or jumping techniques to recover the genomic DNA which confers nematode resistance, since this DNA resides immediately adjacent to the Apase-1 1 gene on chromosome 6 of Lycopersicon esculentum.
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