| abstract |
Improved RP-HPLC methods for purifying recombinant betainterferon are disclosed. Said RP-HPLC methods employ wide pore silica gel reverse-phase columns and solvent systems containing acetonitrile as the organic modifier and either heptafluorobutyric acid or trifluoroacetic acid as the organic acid. n The invention further concerns processes for purifying recombinant IFN-β incorporating said improved RP-HPLC methods. n The invention further concerns recombinant IFN-β purified by said RP-HPLC methods, or recovered and/or purified by processes incorporating said RP-HPLC. |