| abstract |
PCT No. PCT/GB83/00058 Sec. 371 Date Oct. 27, 1983 Sec. 102(e) Date Oct. 27, 1983 PCT Filed Feb. 25, 1983 PCT Pub. No. WO83/03104 PCT Pub. Date Sep. 15, 1983.Assays, e.g. immunoassays, based on the chemiluminescent reaction between a peroxidase enzyme, e.g. horse radish peroxidase, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione, e.g. luminol or isoluminol and an oxidant, e.g. hydrogen peroxide or a perborate, are improved by carrying out the reaction in the presence of a 6-hydroxybenzothiazole, especially 6-hydroxybenzothiazole itself or firefly luciferin. Light emission from the reaction is significantly enhanced by the 6-hydroxybenzothiazole. |