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filingDate 1987-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1989-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1989-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4797364-A
titleOfInvention Process for preparing concentrated amounts of lyophilized microorganisms
abstract The process for preparing concentrated amounts of lyophilized micro-organisms employs an ultrafiltration with a supply of nutritive substrates and the supply of a neutralizing agent in the fermenter, and further comprises the following steps: (a) ceasing to supply substrates while cooling; (b) continuing the ultrafiltration until the obtainment of a primary concentrate (40-80 kg dry weight of bacteria/cu. m) and cooling to 15 DEG -20 DEG C.; (c) washing the primary concentrate with mineral salts and amino acids, oligo-elements and vitamins; (d) concentrating by ultrafiltration to 90-220 kg dry weight of bacteria/cu. m; (e) cooling to 5 DEG C. and adding a protector; (f) deep freezing and lyophilizing. The process particularly applies to the preparation of lactic bacteria.
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