| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0d319d0c526e434caeb449c4e217be88 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1133 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 |
| filingDate |
1985-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1988-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccbb7eeaab23c5687d5922f0381bd150 |
| publicationDate |
1988-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-4766205-A |
| titleOfInvention |
Method for isolation of recombinant polypeptides in biologically active forms |
| abstract |
Disclosed are improved methods for orienting polypeptide molecules containing multiple disulfide bonds into their native conformation through (1) treating the polypeptide with (a) a denaturing agent and (b) a reducing agent capable of reductively dissociating cystine disulfide bonds; (2) concurrently reducing the concentration of the reducing agent while forming a stable intermediate by reacting a disulfide containing compound with the reduced cysteine moieties of the polypeptide and; (3) forming native cystine disulfide bonds in the presence of a mild oxidizing/reducing environment while dissociating the disulfide-group polypeptide moeities. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4977248-A
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016103146-A1
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010226925-A1
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| priorityDate |
1985-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |