http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4740465-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa815277e907c9bf61c727388076593c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-832 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
| filingDate | 1985-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1988-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0f90ea65ff9cbb15a9d3586e78b208f |
| publicationDate | 1988-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-4740465-A |
| titleOfInvention | Heat-resistant sarcosine oxidase N and process for producing the same |
| abstract | When a microorganism belonging to the genus Bacillus and capable of producing heat-resistant sarcosine oxidase N is cultured in a medium, there can be obtained from the cultured medium, heat-resistant sarcosine oxidase N having the following physico-chemical properties: (a) Action Sarcosine oxidase N catalyses the following enzyme reaction wherein sarcosine is oxidatively decomposed to form glycine, formaldehyde and hydrogen peroxide. Sarcosine+H2O+O2->glycine+formaldehyde+H2O2 (b) Substrate specificity Sarcosine oxidase N has a Km value (Michaelis constant) for sarcosine of 4.7 mM at 37 DEG C. at pH 7.7 (phosphate buffer solution). (c) Optimum pH and stable pH range The optimum pH of sarcosine oxidase N is 6.7 to 10.0 when sarcosine is used as a substrate. The stable pH range is 6.5 to 11.5. (d) Optimum temperature range 45 DEG to 60 DEG C. (e) Heat stability Sarcosine oxidase N retains its enzymatic activity of 98% when treated at 55 DEG C. for 10 minutes and of 75% even when treated at 60 DEG C. for 10 minutes. (f) Molecular weight About 49,000 when measured by gel filtration method using Sephadex G-150. (g) Flavin enzyme protein Sarcosine oxidase N contains 1 mole of covalently bound flavin adenine dinucleotide (FAD) per mole of the enzyme. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4826762-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9401539-A1 |
| priorityDate | 1985-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 363.