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filingDate 1985-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1988-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1988-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4732848-A
titleOfInvention Process for the determination of an immunologically-bindable substance involving a Fab fragment
abstract The present invention provides a process for the determination of an immunologically-bindable substance by immunochemical methods in heterogeneous phase, wherein n (a) a sample solution containing the substance to be determined is incubated with n (b) a solid phase-bound antibody against the bindable substance to be determined n (c) a conjugate of the bindable substance to be determined and a Fab fragment of an antibody directed against a determinable enzyme in a definite amount and n (d) the enzyme which corresponds to the antibody contained in the conjugate (c), n the phases are separated and the enzyme activity is measured in one of the two phases. n The present invention also provides a reagent for the determination of an immunologically-bindable substance, wherein it contains a solid phase-bound antibody against the bindable substance to be determined, a conjugate of the bindable substance to be determined and a Fab fragment of an antibody directed against a determinable enzyme, a determinable enzyme which binds immunologically with the Fab fragment contained in the conjugate and buffer.
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