abstract |
The present invention relates to the regeneration of soybeans. The process comprises the steps of: (a) culturing tissue obtained from a soybean plant on a first medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus and embryoid formation; (b) subculturing the embryoids on a second medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure embryoid maturation; (c) subculturing the embryoids on a third medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot formation, and (e) subculturing the shoots on a fourth medium comprising mineral salts, vitamins and sucrose in an amount sufficient to ensure root formation. |