| abstract |
A method for assaying a compound of the formula <IMAGE> wherein R1 is hydroxyl or amino, or hydrogen if at least one of R2, R3, R4, R5 and R6 is hydroxyl or amino, and R2, R3, R4, R5 and R6 are hydrogen, halogen, lower alkyl, lower alkoxy, amino, substituted amino, hydroxy, carboxyl or sulfo, or R5 and R6 together form a ring, comprises establishing a reaction system containing the compound to be assayed and a coupler and an enzyme capable of consuming oxygen and generating a pigment in the presence of the compound and the coupler. A detectable change in the reaction system is measured, to assay the compound in question. The measurement can comprise the measurement of consumed oxygen, as by an oxygen electrode. Or the measurement can comprise the measurement of the generated pigment, as by a colorimetric assay at a specific absorption wavelength thereof. |